Presence or Absence of Significant HPVE4 Expression in High-grade Anal Intraepithelial Neoplasia With p16/Ki-67 Positivity Indicates Distinct Patterns of Neoplasia: A Study Combining Immunohistochemistry and Laser Capture Microdissection PCR.
August 7, 2020

Presence or Absence of Significant HPVE4 Expression in High-grade Anal Intraepithelial Neoplasia With p16/Ki-67 Positivity Indicates Distinct Patterns of Neoplasia: A Study Combining Immunohistochemistry and Laser Capture Microdissection PCR.

By Henry

The development of anal intraepithelial neoplasia (AIN) involves a transition from productive to change papillomavirus (HPV) infection in humans. Grading aims at distinguishing low-grade AIN productive of high-grade anal intraepithelial neoplasia (HGAIN) with cancer risk. We describe the immunohistochemical pattern in AIN add a new marker for the initiation of productive phase of the life cycle of HPV (panHPVE4) to them for activities of the cell cycle (Ki-67) and activity change HPVE7 gene (p16). We studied 67 biopsies for suspected anal anal neoplasia (17 normal, 15 AIN1, AIN2 20, 15 AIN3) of the 54 men who have sex with men in New York Presbyterian Hospital, USA.

Two pathologists who generated consensus and immunogrades AIN. entire network and laser capture microdissection samples of HPV-infected multiple biopsies were tested for HPV with SPF10-PCR-LiPA25 Deia, version 1. (The) basal Ki-67 expression normal differentiated from AIN (≥lower third of Ki-67) with a sensitivity of 0.92 and specificity of 1.0. Ki-67 does not distinguish the value of the AIN. Null / patchwork p16 compared to diffuse pattern of discrimination HGAIN third ≥lower (sensitivity, 1.0; specificity, 0.84). There was marked heterogeneity in expression in HGAIN E4.

Most AIN2 (14/20) is compared to 0/15 AIN3 E4 (sensitivity, 0.70; specificity 1.0). HPV was detected in 63 (94%) biopsies, with 49 (77.8%) high-risk HPV. HPV16 was the most frequent (13%). Some HPV genotype was found in 15 (24%) biopsy and laser capture microdissection -polymerase chain reaction confirmed the specific HPV types in the E4 +/- AIN. Although Ki-67 and p16 HGAIN discrimination AIN, E4 / p16 staining showed that most AIN2 different from AIN3 transformed in the second show went into productive HPV infection and change activity.

Presence or Absence of Significant HPVE4 Expression in High-grade Anal Intraepithelial Neoplasia With p16/Ki-67 Positivity Indicates Distinct Patterns of Neoplasia: A Study Combining Immunohistochemistry and Laser Capture Microdissection PCR.
Presence or Absence of Significant HPVE4 Expression in High-grade Anal Intraepithelial Neoplasia With p16/Ki-67 Positivity Indicates Distinct Patterns of Neoplasia: A Study Combining Immunohistochemistry and Laser Capture Microdissection PCR.

The absence of Cytomegalovirus in Glioblastoma and Other High-grade gliomas by Real-time PCR, Immunohistochemistry and In Situ Hybridization.

Objective: Report of cytomegalovirus (CMV) in the detection of high-grade glioma (HGG) / glioblastoma has been contradictory. We conducted a comprehensive approach to determine the presence or absence of CMV in tissue, plasma, and serum HGG patients.Experimental Design: In a retrospective arm, 25 fresh frozen tissue from glioblastoma patients were tested for CMV by real-time PCR.

Tissue microarray of 70 patients with HGG were tested by IHC and 20 formalin-fixed paraffin-embedded (FFPE) tissue glioblastoma by IHC and chromogenic in situ hybridization (CISH), targeting-encoded CMV IE1 / 2 and pp65. In a prospective arm, 18 newly diagnosed patients provided blood and tissue HGG samples.

Results: All retrospectively collected tissue negative for CMV by all methods. In a prospective cohort, 18 patients newly diagnosed with HGG give a blood sample at the time of diagnosis and during follow-up. Of the 38 specimens of plasma CMV DNA was detected in 3 of 18 samples at the beginning and one of the 20 samples of follow-up. CMV IgG serum positive in 8 of 15 (53%) of the patients. Among the FFPE samples were tested in a prospective arms, were all negative for CMV by IHC, CISH, and PCR.

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Conclusions: 6 Utilizing a highly sensitive test with three orthogonal technology in some specimens and specimen types, there is no evidence of CMV in glioblastoma tissue is found. Our findings call for blind multicenter analysis of samples collected from different geographical regions agreed with the study design and the determination of causality or absence of CMV in HGG / glioblastoma for future guidance on antiviral therapy and / or CMV-based required.