Molecular analysis of the t(2;8)/MYC-IGK translocation in high-grade lymphoma/leukemia by long-distance inverse PCR.

Burkitt’s lymphoma and a subset of diffuse large B-cell lymphoma that is characterized by chromosomal changes affecting the MYC oncogene at 8q24. In most cases the MYC was found juxtaposed with the immunoglobulin heavy chain (IGH) gene locus. Translocation to the immunoglobulin kappa locus (IGK) gene on the 2p11 were observed in approximately 5-10% of cases. few data exist on the molecular mechanisms that lead to these irregularities.

The chromosomal breakpoints on chromosome 8 have been found scattered over a large area 3 ‘of MYC. In order to gain a better understanding of the chromosomal translocations we developed an inverse distance method (LDI) PCR for identification of chromosomal translocations affecting the IGK locus. We investigated a number of samples of lymphoma cytogenetically largely uncharacterized high quality and identify MYC-IGK alignment in seven patients and three t (2; 8) line-positive cells.

Characterized molecular chromosomal breakpoints and analyzed. Linear distance of breakpoints on chromosome 8 for MYC range from some 100 bp to more than 0.5 MB. Reciprocal translocation allele can be characterized in most cases. This study represents the largest series of t (2; 8) positive cases analyzed so far. LDI PCR method developed here should also be useful for the analysis of chromosomal translocation affecting the IGK locus in general.

In a population of outpatients this, the test results hrHPV showed good agreement between the self-sampling and physicians-taken scratches cervical (86%, k = 0.70), with a sensitivity and specificity for CIN2 + that did not differ significantly (93% and 51%, 91% and 51%, respectively (P = 1.0)). Clinical Sensitivity brush-based self-sampling combined with the GP5 + / 6 + -PCR hrHPV EIA test for detecting CIN2 + was non-inferior to that hrHPV testing in cervical samples taken doctors (P = 0.018). Additionally, hrHPV genotype result that fits between sample types, with almost perfect agreement for HPV16 (k = 0.81) and HPV18 (k = 0.92). Finally, 91% of participants described based self-sampling brush is easy to use.

Dynamic active microorganisms inhabiting the industry heap bioleaching of low grade sulphide copper ore monitored by real-time PCR and oligonucleotide microarray prokaryotic acidophile.

The metal sulfide bioleaching has developed into an important industrial process and understand the dynamics of microbes is key to advancing commercial bioleaching operation. Here we report the first quantitative description of the dynamics of the community who are active in the industry heap bioleaching.

Acidithiobacillus ferrooxidans is most abundant during the first part of a washing cycle, while the abundance Leptospirillum ferriphilum and Ferroplasma acidiphilum pile increases with age. Acidithiobacillus thiooxidans remained constant throughout the wash cycle, and Firmicutes group showed low and uneven distribution in the stack. Acidiphilium-like bacteria reached highest abundance in accordance with the number of autotrof.

Active microorganisms in the washing system is determined by using RNA-based two sensitive technique. In most cases, 16S rRNA At copy numbers. ferrooxidans, L. ferriphilum, At. thiooxidans and F. acidiphilum, is in conjunction with DNA copy number, while Acidiphilium as Firmicutes bacteria and some members did not show a clear correlation between the accumulation of 16S rRNA and DNA copy number.

Water, Cell Culture Grade
CA018-600	GenDepot	6x1000ml	EUR 124

Water, PCR Certified
W0806-010	GenDepot	100ml	EUR 88

Water, PCR Certified
W0806-050	GenDepot	500ml	EUR 112

Water, PCR Certified
W0806-100	GenDepot	10x10ml	EUR 97

Water, Ultrapure Molecular Biology Grade
41024-4L	Biotium	4L	EUR 121
Description: Minimum order quantity: 1 unit of 4L

PCR Water 10x1.8 ml
BA01201	Bioatlas	10x1.8ml	EUR 87
Description: PCR grade ultra pure water.

PCR Water 25x1.8 ml
BA01202	Bioatlas	25x1.8ml	EUR 123
Description: PCR grade ultra pure water.

PCR Water 5x10 ml
BA01203	Bioatlas	5x10ml	EUR 123
Description: PCR grade ultra pure water.

4 L REAGENT GRADE WATER, CUBE
46-002-LF	CORNING	4 L/pk	EUR 91
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

Water, BP, Ph. Eur. grade, sterile
GK0904-1L	Glentham Life Sciences	1 l	EUR 74

Cell Culture Grade Water, Cell Culture Tested, Sterile
CCM1081-500	Bio Basic	500 mL	EUR 61.24

Cell Culture Grade Water, Cell Culture Tested, Sterile
CCM2081-1000	Bio Basic	1000 mL	EUR 68.87

100 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS
46-000-CI	CORNING	100 mL/pk	EUR 57
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

1 L, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS
46-000-CM	CORNING	1L/pk	EUR 98
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

500 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS
46-000-CV	CORNING	500 mL/pk	EUR 106
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

100 ML, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CI	CORNING	100 mL/pk	EUR 58
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

1 L, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CM	CORNING	1 L/pk	EUR 72
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

500 ML, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CV	CORNING	500 mL/pk	EUR 59
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

20 L, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-LB	CORNING	20 L/pk	EUR 210
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

100 L, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-LG	CORNING	100 L/pk	EUR 792
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

200 L,CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-LH	CORNING	200 L/pk	EUR 1159
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

Water
C8801	Bio Basic	500ml	EUR 66.97

HyAgarose? HR Agarose, PCR Grade, 100g
R9012HR-100g	ACTGene		EUR 332

HyAgarose? HR Agarose, PCR Grade, 10g
R9012HR-10g	ACTGene		EUR 83

HyAgarose? HR Agarose, PCR Grade, 500g
R9012HR-500g	ACTGene		EUR 1141

HyAgarose? HR Agarose, PCR Grade, 50g
R9012HR-50g	ACTGene		EUR 202

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 572

500 ML, CELL CULTURE GRADE WATER , WITH SEPTUM CAP; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CVC	CORNING	500 mL/pk	EUR 65
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech	50T	EUR 453

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech	100T	EUR 572

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 453

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 572

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 823

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 859

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 823

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 1113

Astrovirus PCR kit
PCR-H491-48R	Bioingentech	50T	EUR 823

Astrovirus PCR kit
PCR-H491-96R	Bioingentech	100T	EUR 1113

Calicivirus PCR kit
PCR-H493-48R	Bioingentech	50T	EUR 823

Calicivirus PCR kit
PCR-H493-96R	Bioingentech	100T	EUR 1113

Adenovirus PCR kit
PCR-H501-48D	Bioingentech	50T	EUR 453

Adenovirus PCR kit
PCR-H501-96D	Bioingentech	100T	EUR 572

Polyomavirus PCR kit
PCR-H515-48D	Bioingentech	50T	EUR 453

Polyomavirus PCR kit
PCR-H515-96D	Bioingentech	100T	EUR 572

Ruminococcus PCR kit
PCR-H619-48D	Bioingentech	48T	EUR 453

Ruminococcus PCR kit
PCR-H619-96D	Bioingentech	96T	EUR 572

Bifidobacterium PCR kit
PCR-H621-48D	Bioingentech	48T	EUR 453

Bifidobacterium PCR kit
PCR-H621-96D	Bioingentech	96T	EUR 572

Sapovirus PCR kit
PCR-H630-48R	Bioingentech	50T	EUR 823

Sapovirus PCR kit
PCR-H630-96R	Bioingentech	100T	EUR 1113

Parechovirus PCR kit
PCR-H642-48R	Bioingentech	50T	EUR 678

Parechovirus PCR kit
PCR-H642-96R	Bioingentech	100T	EUR 1113

Metapneumovirus PCR kit
PCR-H643-48R	Bioingentech	50T	EUR 823

Metapneumovirus PCR kit
PCR-H643-96R	Bioingentech	100T	EUR 1113

Entamoeba PCR kit
PCR-H683-48D	Bioingentech	50T	EUR 453

Entamoeba PCR kit
PCR-H683-96D	Bioingentech	100T	EUR 572

Acanthamoeba PCR kit
PCR-H684-48D	Bioingentech	50T	EUR 453

Acanthamoeba PCR kit
PCR-H684-96D	Bioingentech	100T	EUR 572

Echovirus PCR kit
PCR-H722-48D	Bioingentech	50T	EUR 453

Echovirus PCR kit
PCR-H722-96D	Bioingentech	100T	EUR 572

ranavirus PCR kit
PCR-V337-48D	Bioingentech	50T	EUR 453

ranavirus PCR kit
PCR-V337-96D	Bioingentech	100T	EUR 572

Ruminococcus PCR kit
PCR-V619-48D	Bioingentech	50T	EUR 425.8
Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit
PCR-V619-96D	Bioingentech	100T	EUR 521.5
Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit
PCR-V621-48D	Bioingentech	50T	EUR 425.8
Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit
PCR-V621-96D	Bioingentech	100T	EUR 521.5
Description: An conventional PCR kit for detection of Bifidobacterium

PEPTONE WATER
P16-103-10kg	Alphabiosciences	10 kg	EUR 666

PEPTONE WATER
P16-103-2Kg	Alphabiosciences	2 Kg	EUR 184

PEPTONE WATER
P16-103-500g	Alphabiosciences	500 g	EUR 86

DEPC Water
DD1005(D0121)	Bio Basic	500ml	EUR 61.31

Water Phenol
abx082109-250ml	Abbexa	250 ml	EUR 189

Water, distilled
GK8002-1L	Glentham Life Sciences	1 l	EUR 38

Water, distilled
GK8002-5L	Glentham Life Sciences	5 l	EUR 54

Distilled Water
W0325-050	GenDepot	500 ml	EUR 64

Distilled Water
W0325-100	GenDepot	1L	EUR 69

Water, deionized
WC8800	Bio Basic	4L	EUR 84.36

Water, autoclaved
WW1001	Bio Basic	4L	EUR 69.79

48 WELL PCR MICROPLATE
PCR-48-C	CORNING	10/pk	EUR 102
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Microthrix parvicella PCR kit
PCR-A575-48D	Bioingentech	50T	EUR 453

Microthrix parvicella PCR kit
PCR-A575-96D	Bioingentech	100T	EUR 572

Yersinia pseudotuberculosis PCR kit
PCR-H190-48D	Bioingentech	50T	EUR 453

Yersinia pseudotuberculosis PCR kit
PCR-H190-96D	Bioingentech	100T	EUR 572

Neisseria meningitidis PCR kit
PCR-H421-48D	Bioingentech	50T	EUR 453

Neisseria meningitidis PCR kit
PCR-H421-96D	Bioingentech	100T	EUR 572

Neisseria sp. PCR kit
PCR-H422-48D	Bioingentech	50T	EUR 453

Neisseria sp. PCR kit
PCR-H422-96D	Bioingentech	100T	EUR 572

Haemophilus influenzae PCR kit
PCR-H423-48D	Bioingentech	50T	EUR 453

Haemophilus influenzae PCR kit
PCR-H423-96D	Bioingentech	100T	EUR 572

Trypanosoma cruzi PCR kit
PCR-H425-48D	Bioingentech	50T	EUR 453

Trypanosoma cruzi PCR kit
PCR-H425-96D	Bioingentech	100T	EUR 572

Cronobacter sakazakii PCR kit
PCR-H431-48D	Bioingentech	50T	EUR 453

Cronobacter sakazakii PCR kit
PCR-H431-96D	Bioingentech	100T	EUR 572

Clostridium botulinum PCR kit
PCR-H432-48D	Bioingentech	50T	EUR 453

Clostridium botulinum PCR kit
PCR-H432-96D	Bioingentech	100T	EUR 572

Virus Zika PCR kit
PCR-H434-48R	Bioingentech	50T	EUR 823

Virus Zika PCR kit
PCR-H434-96R	Bioingentech	100T	EUR 1113

Corynebacterium diphtheriae PCR kit
PCR-H435-48D	Bioingentech	50T	EUR 453

Corynebacterium diphtheriae PCR kit
PCR-H435-96D	Bioingentech	100T	EUR 572

Legionella pneumophila PCR kit
PCR-H436-48D	Bioingentech	50T	EUR 453

Legionella pneumophila PCR kit
PCR-H436-96D	Bioingentech	100T	EUR 572

Bordetella pertussis PCR kit
PCR-H437-48D	Bioingentech	50T	EUR 453

Bordetella pertussis PCR kit
PCR-H437-96D	Bioingentech	100T	EUR 572

Bordetella parapertussis PCR kit
PCR-H438-48D	Bioingentech	50T	EUR 453

Bordetella parapertussis PCR kit
PCR-H438-96D	Bioingentech	100T	EUR 572

Chlamydia pneumoniae PCR kit
PCR-H439-48D	Bioingentech	50T	EUR 453

Chlamydia pneumoniae PCR kit
PCR-H439-96D	Bioingentech	100T	EUR 572

Pneumocystis jiroveci PCR kit
PCR-H440-48D	Bioingentech	50T	EUR 453

Pneumocystis jiroveci PCR kit
PCR-H440-96D	Bioingentech	100T	EUR 572

Actinobacillus actinomycetemcomitans PCR kit
PCR-H446-48D	Bioingentech	50T	EUR 453

Actinobacillus actinomycetemcomitans PCR kit
PCR-H446-96D	Bioingentech	100T	EUR 572

Bacteroides fragilis PCR kit
PCR-H447-48D	Bioingentech	50T	EUR 453

Bacteroides fragilis PCR kit
PCR-H447-96D	Bioingentech	100T	EUR 572

Candida sp. PCR kit
PCR-H448-48D	Bioingentech	50T	EUR 453

Candida sp. PCR kit
PCR-H448-96D	Bioingentech	100T	EUR 572

Enterococcus sp. PCR kit
PCR-H449-48D	Bioingentech	50T	EUR 453

Enterococcus sp. PCR kit
PCR-H449-96D	Bioingentech	100T	EUR 572

Neisseria gonorrhoeae PCR kit
PCR-H450-48D	Bioingentech	50T	EUR 453

Neisseria gonorrhoeae PCR kit
PCR-H450-96D	Bioingentech	100T	EUR 572

Dengue virus PCR kit
PCR-H451-48R	Bioingentech	50T	EUR 823

Dengue virus PCR kit
PCR-H451-96R	Bioingentech	100T	EUR 1113

Chlamydia trachomatis PCR kit
PCR-H452-48D	Bioingentech	50T	EUR 453

Chlamydia trachomatis PCR kit
PCR-H452-96D	Bioingentech	100T	EUR 572

Human papillomavirus PCR kit
PCR-H454-48D	Bioingentech	50T	EUR 453

Human papillomavirus PCR kit
PCR-H454-96D	Bioingentech	100T	EUR 572

Parvovirus В19 PCR kit
PCR-H455-48D	Bioingentech	50T	EUR 453

Parvovirus В19 PCR kit
PCR-H455-96D	Bioingentech	100T	EUR 572

Treponema pallidum PCR kit
PCR-H456-48D	Bioingentech	50T	EUR 453

Treponema pallidum PCR kit
PCR-H456-96D	Bioingentech	100T	EUR 572

Trichomona vaginalis PCR kit
PCR-H457-48D	Bioingentech	50T	EUR 453

Trichomona vaginalis PCR kit
PCR-H457-96D	Bioingentech	100T	EUR 572

Ureaplasma parvum PCR kit
PCR-H458-48D	Bioingentech	50T	EUR 453

Ureaplasma parvum PCR kit
PCR-H458-96D	Bioingentech	100T	EUR 572

Ureaplasma sp PCR kit
PCR-H459-48D	Bioingentech	50T	EUR 453

Ureaplasma sp PCR kit
PCR-H459-96D	Bioingentech	100T	EUR 572

Ureaplasma urealyticum PCR kit
PCR-H460-48D	Bioingentech	50T	EUR 453

Ureaplasma urealyticum PCR kit
PCR-H460-96D	Bioingentech	100T	EUR 572

Rotavirus A PCR kit
PCR-H480-48R	Bioingentech	50T	EUR 823

Rotavirus A PCR kit
PCR-H480-96R	Bioingentech	100T	EUR 1113

Rubella virus PCR kit
PCR-H481-48R	Bioingentech	50T	EUR 823

Rubella virus PCR kit
PCR-H481-96R	Bioingentech	100T	EUR 1113

Cryptosporidium parvum PCR kit
PCR-H486-48D	Bioingentech	50T	EUR 453

Cryptosporidium parvum PCR kit
PCR-H486-96D	Bioingentech	100T	EUR 572

Shigella sp. PCR kit
PCR-H488-48D	Bioingentech	50T	EUR 453

Shigella sp. PCR kit
PCR-H488-96D	Bioingentech	100T	EUR 572

Vibrio cholerae PCR kit
PCR-H489-48D	Bioingentech	50T	EUR 453

Vibrio cholerae PCR kit
PCR-H489-96D	Bioingentech	100T	EUR 572

Bacillus cereus PCR kit
PCR-H492-48D	Bioingentech	50T	EUR 453

Bacillus cereus PCR kit
PCR-H492-96D	Bioingentech	100T	EUR 572

Actinomyces israelii PCR kit
PCR-H502-48D	Bioingentech	50T	EUR 453

Actinomyces israelii PCR kit
PCR-H502-96D	Bioingentech	100T	EUR 572

Brucella suis PCR kit
PCR-H503-48D	Bioingentech	50T	EUR 453

Brucella suis PCR kit
PCR-H503-96D	Bioingentech	100T	EUR 572

Bacillus anthracis PCR kit
PCR-H504-48D	Bioingentech	50T	EUR 453

Bacillus anthracis PCR kit
PCR-H504-96D	Bioingentech	100T	EUR 572

Ehrlichia chaffeensis PCR kit
PCR-H505-48D	Bioingentech	50T	EUR 453

Ehrlichia chaffeensis PCR kit
PCR-H505-96D	Bioingentech	100T	EUR 572

Bartonella quintana PCR kit
PCR-H507-48D	Bioingentech	50T	EUR 453

Bartonella quintana PCR kit
PCR-H507-96D	Bioingentech	100T	EUR 572

Eikenella corrodens PCR kit
PCR-H508-48D	Bioingentech	50T	EUR 453

Eikenella corrodens PCR kit
PCR-H508-96D	Bioingentech	100T	EUR 572

However, acidophile microarray (PAM) prokaryotic analysis indicates an active member of Alphaproteobacteria in all samples and of the genus in the older Sulfobacillus. Also, the new active group such as Actinobacteria and Acidobacterium genus detected by PAM. The results showed that changes during the wash cycle in the chemical and physical conditions, such as pH and Fe (3 +) / Fe (2+) ion levels, is a major factor shaping the dynamics of microbes in the pile.