Molecular analysis of the t(2;8)/MYC-IGK translocation in high-grade lymphoma/leukemia by long-distance inverse PCR.

Burkitt’s lymphoma and a subset of diffuse large B-cell lymphoma that is characterized by chromosomal changes affecting the MYC oncogene at 8q24. In most cases the MYC was found juxtaposed with the immunoglobulin heavy chain (IGH) gene locus. Translocation to the immunoglobulin kappa locus (IGK) gene on the 2p11 were observed in approximately 5-10% of cases. few data exist on the molecular mechanisms that lead to these irregularities.

The chromosomal breakpoints on chromosome 8 have been found scattered over a large area 3 ‘of MYC. In order to gain a better understanding of the chromosomal translocations we developed an inverse distance method (LDI) PCR for identification of chromosomal translocations affecting the IGK locus. We investigated a number of samples of lymphoma cytogenetically largely uncharacterized high quality and identify MYC-IGK alignment in seven patients and three t (2; 8) line-positive cells.

Characterized molecular chromosomal breakpoints and analyzed. Linear distance of breakpoints on chromosome 8 for MYC range from some 100 bp to more than 0.5 MB. Reciprocal translocation allele can be characterized in most cases. This study represents the largest series of t (2; 8) positive cases analyzed so far. LDI PCR method developed here should also be useful for the analysis of chromosomal translocation affecting the IGK locus in general.

In a population of outpatients this, the test results hrHPV showed good agreement between the self-sampling and physicians-taken scratches cervical (86%, k = 0.70), with a sensitivity and specificity for CIN2 + that did not differ significantly (93% and 51%, 91% and 51%, respectively (P = 1.0)). Clinical Sensitivity brush-based self-sampling combined with the GP5 + / 6 + -PCR hrHPV EIA test for detecting CIN2 + was non-inferior to that hrHPV testing in cervical samples taken doctors (P = 0.018). Additionally, hrHPV genotype result that fits between sample types, with almost perfect agreement for HPV16 (k = 0.81) and HPV18 (k = 0.92). Finally, 91% of participants described based self-sampling brush is easy to use.

Dynamic active microorganisms inhabiting the industry heap bioleaching of low grade sulphide copper ore monitored by real-time PCR and oligonucleotide microarray prokaryotic acidophile.

The metal sulfide bioleaching has developed into an important industrial process and understand the dynamics of microbes is key to advancing commercial bioleaching operation. Here we report the first quantitative description of the dynamics of the community who are active in the industry heap bioleaching.

Acidithiobacillus ferrooxidans is most abundant during the first part of a washing cycle, while the abundance Leptospirillum ferriphilum and Ferroplasma acidiphilum pile increases with age. Acidithiobacillus thiooxidans remained constant throughout the wash cycle, and Firmicutes group showed low and uneven distribution in the stack. Acidiphilium-like bacteria reached highest abundance in accordance with the number of autotrof.

Active microorganisms in the washing system is determined by using RNA-based two sensitive technique. In most cases, 16S rRNA At copy numbers. ferrooxidans, L. ferriphilum, At. thiooxidans and F. acidiphilum, is in conjunction with DNA copy number, while Acidiphilium as Firmicutes bacteria and some members did not show a clear correlation between the accumulation of 16S rRNA and DNA copy number.

Water (Molecular Biology Grade)
CE243	GeneOn	500 ml	EUR 52

Water (Molecular Biology Grade)
CE244	GeneOn	1 l	EUR 56

Water, PCR Certified
W0806-010	GenDepot	100ml	EUR 88

Water, PCR Certified
W0806-050	GenDepot	500ml	EUR 112

Water, PCR Certified
W0806-100	GenDepot	10x10ml	EUR 97

Water, Ultrapure Molecular Biology Grade
41024-4L	Biotium	4L	EUR 121
Description: Minimum order quantity: 1 unit of 4L

PCR Water 10x1.8 ml
BA01201	Bioatlas	10x1.8ml	EUR 87
Description: PCR grade ultra pure water.

PCR Water 25x1.8 ml
BA01202	Bioatlas	25x1.8ml	EUR 123
Description: PCR grade ultra pure water.

PCR Water 5x10 ml
BA01203	Bioatlas	5x10ml	EUR 123
Description: PCR grade ultra pure water.

4 L REAGENT GRADE WATER, CUBE
46-002-LF	CORNING	4 L/pk	EUR 91
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

Water, BP, Ph. Eur. grade, sterile
GK0904-1L	Glentham Life Sciences	1 l	EUR 74

Cell Culture Grade Water, Cell Culture Tested, Sterile
CCM1081-500	Bio Basic	500 mL	EUR 61.24
Product category: Culture Media

Cell Culture Grade Water, Cell Culture Tested, Sterile
CCM2081-1000	Bio Basic	1000 mL	EUR 68.87
Product category: Culture Media

100 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS
46-000-CI	CORNING	100 mL/pk	EUR 57
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

1 L, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS
46-000-CM	CORNING	1L/pk	EUR 98
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

500 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS
46-000-CV	CORNING	500 mL/pk	EUR 106
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

100 ML, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CI	CORNING	100 mL/pk	EUR 58
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

1 L, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CM	CORNING	1 L/pk	EUR 72
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

500 ML, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CV	CORNING	500 mL/pk	EUR 59
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

20 L, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-LB	CORNING	20 L/pk	EUR 210
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

100 L, CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-LG	CORNING	100 L/pk	EUR 792
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

200 L,CELL CULTURE GRADE WATER; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-LH	CORNING	200 L/pk	EUR 1159
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

HyAgarose? HR Agarose, PCR Grade, 100g
R9012HR-100g	ACTGene		EUR 332

HyAgarose? HR Agarose, PCR Grade, 10g
R9012HR-10g	ACTGene		EUR 83

HyAgarose? HR Agarose, PCR Grade, 500g
R9012HR-500g	ACTGene		EUR 1141

HyAgarose? HR Agarose, PCR Grade, 50g
R9012HR-50g	ACTGene		EUR 202

Water
C8801	Bio Basic	500ml	EUR 66.97
Product category: Culture Media/Water - Culture Grade

500 ML, CELL CULTURE GRADE WATER , WITH SEPTUM CAP; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS
25-055-CVC	CORNING	500 mL/pk	EUR 65
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

Water Phenol
abx082109-250ml	Abbexa	250 ml	EUR 189
Shipped within 5-10 working days.

Water, distilled
GK8002-1L	Glentham Life Sciences	1 l	EUR 38

Water, distilled
GK8002-5L	Glentham Life Sciences	5 l	EUR 54

PEPTONE WATER
P16-103-10kg	Alphabiosciences	10 kg	EUR 666

PEPTONE WATER
P16-103-2Kg	Alphabiosciences	2 Kg	EUR 184

PEPTONE WATER
P16-103-500g	Alphabiosciences	500 g	EUR 86

Distilled Water
W0325-050	GenDepot	500 ml	EUR 64

Distilled Water
W0325-100	GenDepot	1L	EUR 69

DEPC Water
DD1005(D0121)	Bio Basic	500ml	EUR 61.31
Product category: Biochemicals/Biology Reagents/RNA Related

Water, deionized
WC8800	Bio Basic	4L	EUR 84.36
Product category: Culture Media/Water - Culture Grade

Water, autoclaved
WW1001	Bio Basic	4L	EUR 69.79
Product category: Culture Media/Water - Culture Grade

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Halobacillus

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Halobacillus

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Enterovirus

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Enterovirus

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Mengovirus

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Mengovirus

Astrovirus PCR kit
PCR-H491-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Astrovirus

Astrovirus PCR kit
PCR-H491-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Astrovirus

Calicivirus PCR kit
PCR-H493-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Calicivirus

Calicivirus PCR kit
PCR-H493-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Calicivirus

Adenovirus PCR kit
PCR-H501-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Adenovirus

Adenovirus PCR kit
PCR-H501-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Adenovirus

Polyomavirus PCR kit
PCR-H515-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Polyomavirus

Polyomavirus PCR kit
PCR-H515-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Polyomavirus

Sapovirus PCR kit
PCR-H630-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Sapovirus

Sapovirus PCR kit
PCR-H630-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Sapovirus

Parechovirus PCR kit
PCR-H642-48R	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Parechovirus

Parechovirus PCR kit
PCR-H642-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Parechovirus

Metapneumovirus PCR kit
PCR-H643-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Metapneumovirus

Metapneumovirus PCR kit
PCR-H643-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Metapneumovirus

Entamoeba PCR kit
PCR-H683-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Entamoeba

Entamoeba PCR kit
PCR-H683-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Entamoeba

Acanthamoeba PCR kit
PCR-H684-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Acanthamoeba

Acanthamoeba PCR kit
PCR-H684-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Acanthamoeba

Echovirus PCR kit
PCR-H722-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Echovirus

Echovirus PCR kit
PCR-H722-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Echovirus

ranavirus PCR kit
PCR-V337-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of ranavirus

ranavirus PCR kit
PCR-V337-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of ranavirus

Ruminococcus PCR kit
PCR-V619-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit
PCR-V619-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit
PCR-V621-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit
PCR-V621-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Bifidobacterium

Hantavirus PCR kit
PCR-VH373-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Hantavirus

Hantavirus PCR kit
PCR-VH373-96R	Bioingentech	100T	EUR 669.4
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Description: An conventional PCR kit for detection of Hantavirus

Ruminococcus PCR kit
PCR-H619-48D	Bioingentech	48T	EUR 453

Ruminococcus PCR kit
PCR-H619-96D	Bioingentech	96T	EUR 572

Bifidobacterium PCR kit
PCR-H621-48D	Bioingentech	48T	EUR 453

Bifidobacterium PCR kit
PCR-H621-96D	Bioingentech	96T	EUR 572

48 WELL PCR MICROPLATE
PCR-48-C	CORNING	10/pk	EUR 102
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Microthrix parvicella PCR kit
PCR-A575-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Microthrix parvicella

Microthrix parvicella PCR kit
PCR-A575-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Microthrix parvicella

Yersinia pseudotuberculosis PCR kit
PCR-H190-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis

Yersinia pseudotuberculosis PCR kit
PCR-H190-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis

Neisseria meningitidis PCR kit
PCR-H421-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Neisseria meningitidis

Neisseria meningitidis PCR kit
PCR-H421-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Neisseria meningitidis

Neisseria sp. PCR kit
PCR-H422-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Neisseria sp.

Neisseria sp. PCR kit
PCR-H422-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Neisseria sp.

Haemophilus influenzae PCR kit
PCR-H423-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Haemophilus influenzae

Haemophilus influenzae PCR kit
PCR-H423-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Haemophilus influenzae

Trypanosoma cruzi PCR kit
PCR-H425-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Trypanosoma cruzi

Trypanosoma cruzi PCR kit
PCR-H425-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Trypanosoma cruzi

Cronobacter sakazakii PCR kit
PCR-H431-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Cronobacter sakazakii

Cronobacter sakazakii PCR kit
PCR-H431-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Cronobacter sakazakii

Clostridium botulinum PCR kit
PCR-H432-48D	Bioingentech	50T	EUR 425.8
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Description: An conventional PCR kit for detection of Clostridium botulinum

Clostridium botulinum PCR kit
PCR-H432-96D	Bioingentech	100T	EUR 521.5
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Description: An conventional PCR kit for detection of Clostridium botulinum

Virus Zika PCR kit
PCR-H434-48R	Bioingentech	50T	EUR 524.4
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Description: An conventional PCR kit for detection of Virus Zika

However, acidophile microarray (PAM) prokaryotic analysis indicates an active member of Alphaproteobacteria in all samples and of the genus in the older Sulfobacillus. Also, the new active group such as Actinobacteria and Acidobacterium genus detected by PAM. The results showed that changes during the wash cycle in the chemical and physical conditions, such as pH and Fe (3 +) / Fe (2+) ion levels, is a major factor shaping the dynamics of microbes in the pile.