Human papillomavirus detection by PCR assay in a large series of high-grade squamous intraepithelial lesions with cytohistological correlation and follow-up.

OBJECTIVE
High-grade squamous intraepithelial lesions (HSIL) is a precursor of invasive cervical carcinoma and is generally associated with the integration of mucosotropic human papillomavirus (HPV) DNA into the host cell genome. HPV detection is easy to do at this time, even in the laboratory with limited technological capacity, and follow-up procedures for patients with HSIL established.

METHOD
HPV detection is done in a large group of patients with HSIL, and the results were correlated with the findings of cytology, histology, and colposcopy. Differences examined and discussed.

RESULTS
Conventional Papanicolaou (Pap) screening detected 446 HSIL (0.20%) in 218 906 cervical smears. HPV detection by PCR positive in 339/358 (94.7%) patients. Strains involved: HPV 16 in 180 patients (53.1%), HPV 18 in 35 (10.3%), HPV 31/33 in 27 (8%), HPV 6/11 in 10 (2.96%) and of an unknown type in 73 (30%). For the last 97 patients (2006-2007), HPV typing expanded with the following results: HPV 52 was detected in 9 patients (9.2%), HPV 58 in 6 (6.1%), HPV 51 in 4 (4.1 %), HPV 68 in 2 (2.0%), and HPV 39 in 1 (1.0%). The number of patients nonidentified dropped to 9 (9.4%); In addition, 14/97 (14.4%) patients were infected with two or more types of the virus.

Finally, 19 (5.3%) patients with HPV-negative. Colposcopy revealed a small change in 59 patients (17.3%), major changes in 264 (77.6%), and normal findings in 17 (5.1%). A biopsy was taken in 331/446 patients, and the diagnosis of HSIL or overt malignancy was confirmed histologically in 281 (84.9%) patients: in 46 CIN II, CIN III in 224, and histological upgrade in 11 (6 microinvasive squamous carcinoma , 1 squamous carcinoma, 2 endocervical adenocarcinoma in situ, microinvasive endocervical adenocarcinoma and 2). Thirty-five patients (10.6%) relegated to CIN I and 15 (4.5%) patients had negative biopsies. Follow-up in patients with negative biopsies confirmed the existence of SIL in 11 patients [1 HSIL and 10 low-grade squamous intraepithelial lesions (LSIL)] while the fourth is considered a false positive (atrophic changes, 2; changes in reactive, 2).

After treatment, 31/331 (9.36%) patients displayed recurrence (HSIL and LSIL in 29 at 2). Strain of virus involved in patients with relapse were HPV 16 in 16 patients (51.6%); HPV 18 in 4 (12.9%); HPV 16 and 18 in 1 (3.2%); HPV 31 in 1 (3.2%); HPV 52 in 1 (3.2%); HPV 18, 31, and 58 in 1 (3.2%); HPV 68 in 1 (3.2%); HPV 51 and 73 in 1 (3.2%), and of an unknown type in 5 (16.1%). Follow-up in 14/19 HSIL and HPV-negative patients confirmed the existence of cervical pathology.

CONCLUSION
HPV detection improve diagnostic sensitivity and provides an ideal tool for monitoring the response to treatment in patients with HSIL. Pathogenic relevance of HPV strain 18 may be larger than previously thought.

analysis of real-time PCR based on the BRAF V600E mutation in the low and medium grade lymphoma assert common in hairy cell leukemia.

Hairy cell leukemia (HCL) is a rare type of B-cell non-Hodgkin lymphoma (B-NHL), which is not known to be associated with recurrent abnormality karyotype characteristics. A recent study used massively parallel whole exome sequencing to identify mutations in the BRAF V600E enabled, which appears on the HCL.

Here, we confirmed the specificity of BRAF V600E for HCL among low- and middle-grade B-NHL and describes a method of polymerase chain reaction real-time to detect this mutation in cases with a low tumor burden. The V600E mutation does not appear associated with microsatellite instability, unlike the case of colorectal cancer.

Halobacillus PCR kit
PCR-A635-48D	Bioingentech	50T	EUR 453

Halobacillus PCR kit
PCR-A635-96D	Bioingentech	100T	EUR 572

Enterovirus PCR kit
PCR-H433-48R	Bioingentech	50T	EUR 823

Enterovirus PCR kit
PCR-H433-96R	Bioingentech	100T	EUR 859

Mengovirus PCR kit
PCR-H453-48R	Bioingentech	50T	EUR 823

Mengovirus PCR kit
PCR-H453-96R	Bioingentech	100T	EUR 1113

Astrovirus PCR kit
PCR-H491-48R	Bioingentech	50T	EUR 823

Astrovirus PCR kit
PCR-H491-96R	Bioingentech	100T	EUR 1113

Calicivirus PCR kit
PCR-H493-48R	Bioingentech	50T	EUR 823

Calicivirus PCR kit
PCR-H493-96R	Bioingentech	100T	EUR 1113

Adenovirus PCR kit
PCR-H501-48D	Bioingentech	50T	EUR 453

Adenovirus PCR kit
PCR-H501-96D	Bioingentech	100T	EUR 572

Polyomavirus PCR kit
PCR-H515-48D	Bioingentech	50T	EUR 453

Polyomavirus PCR kit
PCR-H515-96D	Bioingentech	100T	EUR 572

Ruminococcus PCR kit
PCR-H619-48D	Bioingentech	48T	EUR 453

Ruminococcus PCR kit
PCR-H619-96D	Bioingentech	96T	EUR 572

Bifidobacterium PCR kit
PCR-H621-48D	Bioingentech	48T	EUR 453

Bifidobacterium PCR kit
PCR-H621-96D	Bioingentech	96T	EUR 572

Sapovirus PCR kit
PCR-H630-48R	Bioingentech	50T	EUR 823

Sapovirus PCR kit
PCR-H630-96R	Bioingentech	100T	EUR 1113

Parechovirus PCR kit
PCR-H642-48R	Bioingentech	50T	EUR 678

Parechovirus PCR kit
PCR-H642-96R	Bioingentech	100T	EUR 1113

Metapneumovirus PCR kit
PCR-H643-48R	Bioingentech	50T	EUR 823

Metapneumovirus PCR kit
PCR-H643-96R	Bioingentech	100T	EUR 1113

Entamoeba PCR kit
PCR-H683-48D	Bioingentech	50T	EUR 453

Entamoeba PCR kit
PCR-H683-96D	Bioingentech	100T	EUR 572

Acanthamoeba PCR kit
PCR-H684-48D	Bioingentech	50T	EUR 453

Acanthamoeba PCR kit
PCR-H684-96D	Bioingentech	100T	EUR 572

Echovirus PCR kit
PCR-H722-48D	Bioingentech	50T	EUR 453

Echovirus PCR kit
PCR-H722-96D	Bioingentech	100T	EUR 572

ranavirus PCR kit
PCR-V337-48D	Bioingentech	50T	EUR 453

ranavirus PCR kit
PCR-V337-96D	Bioingentech	100T	EUR 572

Ruminococcus PCR kit
PCR-V619-48D	Bioingentech	50T	EUR 425.8
Description: An conventional PCR kit for detection of Ruminococcus

Ruminococcus PCR kit
PCR-V619-96D	Bioingentech	100T	EUR 521.5
Description: An conventional PCR kit for detection of Ruminococcus

Bifidobacterium PCR kit
PCR-V621-48D	Bioingentech	50T	EUR 425.8
Description: An conventional PCR kit for detection of Bifidobacterium

Bifidobacterium PCR kit
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Description: An conventional PCR kit for detection of Bifidobacterium

Trypsin, Recombinant, Proteomics grade
P1228-1000	Biovision		EUR 196

Trypsin, Recombinant, Proteomics grade
P1228-10000	Biovision		EUR 1224

Trypsin, Recombinant, Proteomics grade
P1228-5000	Biovision		EUR 615

48 WELL PCR MICROPLATE
PCR-48-C	CORNING	10/pk	EUR 102
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Microthrix parvicella PCR kit
PCR-A575-48D	Bioingentech	50T	EUR 453

Microthrix parvicella PCR kit
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Yersinia pseudotuberculosis PCR kit
PCR-H190-48D	Bioingentech	50T	EUR 453

Yersinia pseudotuberculosis PCR kit
PCR-H190-96D	Bioingentech	100T	EUR 572

Neisseria meningitidis PCR kit
PCR-H421-48D	Bioingentech	50T	EUR 453

Neisseria meningitidis PCR kit
PCR-H421-96D	Bioingentech	100T	EUR 572

Neisseria sp. PCR kit
PCR-H422-48D	Bioingentech	50T	EUR 453

Neisseria sp. PCR kit
PCR-H422-96D	Bioingentech	100T	EUR 572

Haemophilus influenzae PCR kit
PCR-H423-48D	Bioingentech	50T	EUR 453

Haemophilus influenzae PCR kit
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Trypanosoma cruzi PCR kit
PCR-H425-48D	Bioingentech	50T	EUR 453

Trypanosoma cruzi PCR kit
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Cronobacter sakazakii PCR kit
PCR-H431-48D	Bioingentech	50T	EUR 453

Cronobacter sakazakii PCR kit
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Clostridium botulinum PCR kit
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Clostridium botulinum PCR kit
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Virus Zika PCR kit
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Virus Zika PCR kit
PCR-H434-96R	Bioingentech	100T	EUR 1113

Corynebacterium diphtheriae PCR kit
PCR-H435-48D	Bioingentech	50T	EUR 453

Corynebacterium diphtheriae PCR kit
PCR-H435-96D	Bioingentech	100T	EUR 572

Legionella pneumophila PCR kit
PCR-H436-48D	Bioingentech	50T	EUR 453

Legionella pneumophila PCR kit
PCR-H436-96D	Bioingentech	100T	EUR 572

Bordetella pertussis PCR kit
PCR-H437-48D	Bioingentech	50T	EUR 453

Bordetella pertussis PCR kit
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Bordetella parapertussis PCR kit
PCR-H438-48D	Bioingentech	50T	EUR 453

Bordetella parapertussis PCR kit
PCR-H438-96D	Bioingentech	100T	EUR 572

Chlamydia pneumoniae PCR kit
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Chlamydia pneumoniae PCR kit
PCR-H439-96D	Bioingentech	100T	EUR 572

Pneumocystis jiroveci PCR kit
PCR-H440-48D	Bioingentech	50T	EUR 453

Pneumocystis jiroveci PCR kit
PCR-H440-96D	Bioingentech	100T	EUR 572

Actinobacillus actinomycetemcomitans PCR kit
PCR-H446-48D	Bioingentech	50T	EUR 453

Actinobacillus actinomycetemcomitans PCR kit
PCR-H446-96D	Bioingentech	100T	EUR 572

Bacteroides fragilis PCR kit
PCR-H447-48D	Bioingentech	50T	EUR 453

Bacteroides fragilis PCR kit
PCR-H447-96D	Bioingentech	100T	EUR 572

Candida sp. PCR kit
PCR-H448-48D	Bioingentech	50T	EUR 453

Candida sp. PCR kit
PCR-H448-96D	Bioingentech	100T	EUR 572

Enterococcus sp. PCR kit
PCR-H449-48D	Bioingentech	50T	EUR 453

Enterococcus sp. PCR kit
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Neisseria gonorrhoeae PCR kit
PCR-H450-48D	Bioingentech	50T	EUR 453

Neisseria gonorrhoeae PCR kit
PCR-H450-96D	Bioingentech	100T	EUR 572

Dengue virus PCR kit
PCR-H451-48R	Bioingentech	50T	EUR 823

Dengue virus PCR kit
PCR-H451-96R	Bioingentech	100T	EUR 1113

Chlamydia trachomatis PCR kit
PCR-H452-48D	Bioingentech	50T	EUR 453

Chlamydia trachomatis PCR kit
PCR-H452-96D	Bioingentech	100T	EUR 572

Human papillomavirus PCR kit
PCR-H454-48D	Bioingentech	50T	EUR 453

Human papillomavirus PCR kit
PCR-H454-96D	Bioingentech	100T	EUR 572

Parvovirus В19 PCR kit
PCR-H455-48D	Bioingentech	50T	EUR 453

Parvovirus В19 PCR kit
PCR-H455-96D	Bioingentech	100T	EUR 572

Treponema pallidum PCR kit
PCR-H456-48D	Bioingentech	50T	EUR 453

Treponema pallidum PCR kit
PCR-H456-96D	Bioingentech	100T	EUR 572

Trichomona vaginalis PCR kit
PCR-H457-48D	Bioingentech	50T	EUR 453

Trichomona vaginalis PCR kit
PCR-H457-96D	Bioingentech	100T	EUR 572

Ureaplasma parvum PCR kit
PCR-H458-48D	Bioingentech	50T	EUR 453

Ureaplasma parvum PCR kit
PCR-H458-96D	Bioingentech	100T	EUR 572

Ureaplasma sp PCR kit
PCR-H459-48D	Bioingentech	50T	EUR 453

Ureaplasma sp PCR kit
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Ureaplasma urealyticum PCR kit
PCR-H460-48D	Bioingentech	50T	EUR 453

Ureaplasma urealyticum PCR kit
PCR-H460-96D	Bioingentech	100T	EUR 572

Rotavirus A PCR kit
PCR-H480-48R	Bioingentech	50T	EUR 823

Rotavirus A PCR kit
PCR-H480-96R	Bioingentech	100T	EUR 1113

Rubella virus PCR kit
PCR-H481-48R	Bioingentech	50T	EUR 823

Rubella virus PCR kit
PCR-H481-96R	Bioingentech	100T	EUR 1113

Cryptosporidium parvum PCR kit
PCR-H486-48D	Bioingentech	50T	EUR 453

Cryptosporidium parvum PCR kit
PCR-H486-96D	Bioingentech	100T	EUR 572

Shigella sp. PCR kit
PCR-H488-48D	Bioingentech	50T	EUR 453

Shigella sp. PCR kit
PCR-H488-96D	Bioingentech	100T	EUR 572

Vibrio cholerae PCR kit
PCR-H489-48D	Bioingentech	50T	EUR 453

Vibrio cholerae PCR kit
PCR-H489-96D	Bioingentech	100T	EUR 572

Bacillus cereus PCR kit
PCR-H492-48D	Bioingentech	50T	EUR 453

Bacillus cereus PCR kit
PCR-H492-96D	Bioingentech	100T	EUR 572

Actinomyces israelii PCR kit
PCR-H502-48D	Bioingentech	50T	EUR 453

Actinomyces israelii PCR kit
PCR-H502-96D	Bioingentech	100T	EUR 572

Brucella suis PCR kit
PCR-H503-48D	Bioingentech	50T	EUR 453

Brucella suis PCR kit
PCR-H503-96D	Bioingentech	100T	EUR 572

Bacillus anthracis PCR kit
PCR-H504-48D	Bioingentech	50T	EUR 453

Bacillus anthracis PCR kit
PCR-H504-96D	Bioingentech	100T	EUR 572

Ehrlichia chaffeensis PCR kit
PCR-H505-48D	Bioingentech	50T	EUR 453

Ehrlichia chaffeensis PCR kit
PCR-H505-96D	Bioingentech	100T	EUR 572

Bartonella quintana PCR kit
PCR-H507-48D	Bioingentech	50T	EUR 453

Bartonella quintana PCR kit
PCR-H507-96D	Bioingentech	100T	EUR 572

Eikenella corrodens PCR kit
PCR-H508-48D	Bioingentech	50T	EUR 453

Eikenella corrodens PCR kit
PCR-H508-96D	Bioingentech	100T	EUR 572

Fusobacterium nucleatum PCR kit
PCR-H509-48D	Bioingentech	50T	EUR 453

Fusobacterium nucleatum PCR kit
PCR-H509-96D	Bioingentech	100T	EUR 572

Fusobacterium necrophorum PCR kit
PCR-H510-48D	Bioingentech	50T	EUR 453

Fusobacterium necrophorum PCR kit
PCR-H510-96D	Bioingentech	100T	EUR 572

Fusobacterium sp. PCR kit
PCR-H511-48D	Bioingentech	50T	EUR 453

Fusobacterium sp. PCR kit
PCR-H511-96D	Bioingentech	100T	EUR 572

Mycobacterium sp. PCR kit
PCR-H512-48D	Bioingentech	50T	EUR 453

Mycobacterium sp. PCR kit
PCR-H512-96D	Bioingentech	100T	EUR 572

Polyomavirus BK PCR kit
PCR-H513-48D	Bioingentech	50T	EUR 453

Polyomavirus BK PCR kit
PCR-H513-96D	Bioingentech	100T	EUR 572

Polyomavirus JC PCR kit
PCR-H514-48D	Bioingentech	50T	EUR 453

Polyomavirus JC PCR kit
PCR-H514-96D	Bioingentech	100T	EUR 572

Peptostreptococcus anaerobius PCR kit
PCR-H516-48D	Bioingentech	50T	EUR 453

Peptostreptococcus anaerobius PCR kit
PCR-H516-96D	Bioingentech	100T	EUR 572

Proteus sp. PCR kit
PCR-H517-48D	Bioingentech	50T	EUR 453

Proteus sp. PCR kit
PCR-H517-96D	Bioingentech	100T	EUR 572

Streptococcus pyogenes PCR kit
PCR-H518-48D	Bioingentech	50T	EUR 453

Streptococcus pyogenes PCR kit
PCR-H518-96D	Bioingentech	100T	EUR 572

Helicobacter spp. PCR kit
PCR-H520-48D	Bioingentech	50T	EUR 453

Helicobacter spp. PCR kit
PCR-H520-96D	Bioingentech	100T	EUR 572

Chikungunya virus PCR kit
PCR-H525-48R	Bioingentech	50T	EUR 823

Chikungunya virus PCR kit
PCR-H525-96R	Bioingentech	100T	EUR 1113

Yersinia pestis PCR kit
PCR-H604-48D	Bioingentech	50T	EUR 453

Yersinia pestis PCR kit
PCR-H604-96D	Bioingentech	100T	EUR 572

Naegleria fowleri PCR kit
PCR-H611-48D	Bioingentech	50T	EUR 453

Naegleria fowleri PCR kit
PCR-H611-96D	Bioingentech	100T	EUR 572

Vibrio vulnificus PCR kit
PCR-H612-48D	Bioingentech	50T	EUR 453

Vibrio vulnificus PCR kit
PCR-H612-96D	Bioingentech	100T	EUR 572

Bacteroidetes (phylum) PCR kit
PCR-H615-48D	Bioingentech	48T	EUR 453

Bacteroidetes (phylum) PCR kit
PCR-H615-96D	Bioingentech	96T	EUR 572

Faecalibacterium prausnitzii PCR kit
PCR-H617-48D	Bioingentech	48T	EUR 453

Faecalibacterium prausnitzii PCR kit
PCR-H617-96D	Bioingentech	96T	EUR 572

Roseburia sp. PCR kit
PCR-H618-48D	Bioingentech	48T	EUR 453

Roseburia sp. PCR kit
PCR-H618-96D	Bioingentech	96T	EUR 572

Methanobrevibacter smithii PCR kit
PCR-H622-48D	Bioingentech	48T	EUR 453

Methanobrevibacter smithii PCR kit
PCR-H622-96D	Bioingentech	96T	EUR 572

Candida krusei PCR kit
PCR-H629-48D	Bioingentech	50T	EUR 453

Candida krusei PCR kit
PCR-H629-96D	Bioingentech	100T	EUR 572

Giardia lamblia PCR kit
PCR-H633-48D	Bioingentech	50T	EUR 453

Giardia lamblia PCR kit
PCR-H633-96D	Bioingentech	100T	EUR 572

Norovirus G1 PCR kit
PCR-H637-48R	Bioingentech	50T	EUR 823

Norovirus G1 PCR kit
PCR-H637-96R	Bioingentech	100T	EUR 1113

Norovirus G2 PCR kit
PCR-H638-48R	Bioingentech	50T	EUR 823

Norovirus G2 PCR kit
PCR-H638-96R	Bioingentech	100T	EUR 1113

Measles Virus PCR kit
PCR-H639-48R	Bioingentech	50T	EUR 823

Measles Virus PCR kit
PCR-H639-96R	Bioingentech	100T	EUR 1113

Mumps Virus PCR kit
PCR-H640-48R	Bioingentech	50T	EUR 823

Mumps Virus PCR kit
PCR-H640-96R	Bioingentech	100T	EUR 1113

Influenza B PCR kit
PCR-H641-48R	Bioingentech	50T	EUR 823

Influenza B PCR kit
PCR-H641-96R	Bioingentech	100T	EUR 1113

Moraxella catarrhalis PCR kit
PCR-H644-48D	Bioingentech	50T	EUR 453

Moraxella catarrhalis PCR kit
PCR-H644-96D	Bioingentech	100T	EUR 572

Influenza C PCR kit
PCR-H645-48R	Bioingentech	50T	EUR 823

Influenza C PCR kit
PCR-H645-96R	Bioingentech	100T	EUR 1113

Borrelia miyatomoi PCR kit
PCR-H662-48D	Bioingentech	50T	EUR 453

Borrelia miyatomoi PCR kit
PCR-H662-96D	Bioingentech	100T	EUR 572

Mycobacterium complex PCR kit
PCR-H663-48D	Bioingentech	50T	EUR 453

Mycobacterium complex PCR kit
PCR-H663-96D	Bioingentech	100T	EUR 572

Atopobium vaginae PCR kit
PCR-H665-48D	Bioingentech	50T	EUR 453

Atopobium vaginae PCR kit
PCR-H665-96D	Bioingentech	100T	EUR 572

Thus, in conjunction with previous data, our results suggest the incorporation of BRAF V600E mutation analysis in the diagnostic workup of cases HCL. In addition, targeting the Ras-Raf-Mek-Erk-Map kinase should be investigated as a potential therapeutic strategy for patients with this disease.