Category: PCR

August 7, 2020

Human papillomavirus detection by PCR assay in a large series of high-grade squamous intraepithelial lesions with cytohistological correlation and follow-up.

OBJECTIVEHigh-grade squamous intraepithelial lesions (HSIL) is a precursor of invasive cervical carcinoma and is generally associated with the integration of mucosotropic human papillomavirus (HPV) DNA into the host cell genome. HPV detection is easy to do at this time, even in the laboratory with limited technological capacity, and follow-up procedures for patients with HSIL established. METHODHPV detection is done in a large group of patients […]

August 7, 2020

Molecular analysis of the t(2;8)/MYC-IGK translocation in high-grade lymphoma/leukemia by long-distance inverse PCR.

Burkitt’s lymphoma and a subset of diffuse large B-cell lymphoma that is characterized by chromosomal changes affecting the MYC oncogene at 8q24. In most cases the MYC was found juxtaposed with the immunoglobulin heavy chain (IGH) gene locus. Translocation to the immunoglobulin kappa locus (IGK) gene on the 2p11 were observed in approximately 5-10% of cases. few data exist on the molecular mechanisms that lead […]

August 7, 2020

Detecting the H3F3A mutant allele found in high-grade pediatric glioma by real-time PCR.

Diffuse intrinsic pontine glioma (DIPG) is an aggressive pediatric brain tumors with a median survival of 1 year after diagnosis. It has been reported recently that about 80% of cases DIPG and 70% of glioblastomas middle line contained a mutation in one allele of the gene H3F3A (encoding the H3 histone variant H3.3), replacing the lysine 27 to methionine (K27M). In order to facilitate the […]

August 7, 2020

Presence or Absence of Significant HPVE4 Expression in High-grade Anal Intraepithelial Neoplasia With p16/Ki-67 Positivity Indicates Distinct Patterns of Neoplasia: A Study Combining Immunohistochemistry and Laser Capture Microdissection PCR.

The development of anal intraepithelial neoplasia (AIN) involves a transition from productive to change papillomavirus (HPV) infection in humans. Grading aims at distinguishing low-grade AIN productive of high-grade anal intraepithelial neoplasia (HGAIN) with cancer risk. We describe the immunohistochemical pattern in AIN add a new marker for the initiation of productive phase of the life cycle of HPV (panHPVE4) to them for activities of the […]